Ruczinski, Sosnick, and Plaxco Ruczinski I, Sosnick TR, and Plaxco KW.
Methods for the accurate estimation of confidence intervals on experimental Φ-values.

Please read: important file format information for uploading your data. For options and output information, please read the help file.

  1. Upload your data:

  2. Specify the temperature: T = °C

  3. Specify the energy units: kJ kcal

  4. Specify the comparisons: wildtype only all comparisons

  5. Specify the type of fit: individual fits parallel arms

  6. Specify the denaturant concentrations: folding rate: M - unfolding rate: M

  7. Specify the coverage for the Φ-value confidence intervals: % coverage

  8. Specify the number of significant digits in the output: significant digits

  9. And they are off:
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